[i]Conidames[/i], a new oriental genus of Sycophaginae (Hymenoptera, Agaonidae) associated with [i]Ficus[/i] section Conosycea (Moraceae)

The sycophagines are strictly associated with two subgenera of Fit to L. (Moraceae), namely,Slycomo77/S and Urostignza. They mostly oviposit through the fig wall and lay their eggs within the fig flowers, being either gall-makers or parasitoids of other fig wasps. In this contribution, a new genus of Sycophaginae, [i]Conidarnes[/i] Earache & Rasplus, gen. n., is described with seven new species: [i]Conidarnes achterbergi[/i] Earache & Rasplus, sp. n.; [i]Conidarwes beryl'[/i] Farache & Rasplus, sp. n.; [i]Conidarnes laevis[/i] Earache & Rasplus, sp. n.;[i] Conidames santineloi[/i] Earache & Rasplus, sp. n.; [i]Conidarnes subtectae[/i] Earache & Rasplus, sp. n.; [i]Conidarnes sidcata[/i] Farache & Rasplus, sp. n.; and [i]Conidarnes sumatranae[/i] Farache & Rasplus, sp. n. Illustrations, morphological diagnoses, dichotomous keys and multi-entry online keys to species are provided. [i]Conidarnes[/i] species strictly occur in the oriental region, and their distribution does not oVerlap with the distribution of the two other genera belonging to the same clade. Due to their relative rarity, we encourage extensive sampling of Conosycea figs to improve our knowledge of the genus

A PatchMatch-based Dense-field Algorithm for Video Copy-Move Detection and Localization

We propose a new algorithm for the reliable detection and localization of video copy-move forgeries. Discovering well crafted video copy-moves may be very difficult, especially when some uniform background is copied to occlude foreground objects. To reliably detect both additive and occlusive copy-moves we use a dense-field approach, with invariant features that guarantee robustness to several post-processing operations. To limit complexity, a suitable video-oriented version of PatchMatch is used, with a multiresolution search strategy, and a focus on volumes of interest. Performance assessment relies on a new dataset, designed ad hoc, with realistic copy-moves and a wide variety of challenging situations. Experimental results show the proposed method to detect and localize video copy-moves with good accuracy even in adverse conditions

Multilocus phylogeny and ecological differentiation of the "Eupelmus urozonus species group" (Hymenoptera, Eupelmidae) in the West-Palaearctic

Background: The ecological differentiation of insects with parasitic life-style is a complex process that may involve phylogenetic constraints as well as morphological and/ or behavioural adaptations. In most cases, the relative importance of these driving forces remains unexplored. We investigate here this question for the “ Eupelmus urozonus species group ” which encompasses parasitoid wasps of potential interest in biological control. This was achieved using seven molecular markers, re liable records on 91 host species and a proxy of the ovipositor length. Results: After using an adequate partitioning scheme, Maximum likelihood and Bayesian approaches provide a well-resolved phylogeny supporting the monophyly of this species group and highlighting its subdivision into three sub-groups. Great variations of both the ovipositor length and the host range (specialist versus generalist) were observed at this scale, with these two features being not significantly constrained by the phylogeny. Ovipositor length was not shown as a significant predictor of the parasitoid host range. Conclusions: This study provides firstly the first evidence for the strong lability of both the ovipositor's length and the realised host range in a set of phylogeneticall y related and sympatric species. In both cases, strong contrasts were observed between sister species. Moreover, no significant correlation was found between these two features. Alternative drivers of the ecological differentiation such as interspecific interactions are proposed and the consequences on the recruitment of these parasitoids on native and exotic pests are discussed

A multilocus phylogeny of the world Sycoecinae fig wasps (Chalcidoidea: Pteromalidae)

The Sycoecinae is one of five chalcid subfamilies of fig wasps that are mostly dependent on Ficus inflorescences for reproduction. Here, we analysed two mitochondrial ( COI , Cyt b ) and four nuclear genes (ITS2, EF-1α, RpL27a, mago nashi ) from a worldwide sample of 56 sycoecine species. Various alignment and partitioning strategies were used to test the stability of major clades. All topologies estimated using maximum likelihood and Bayesian methods were similar and well resolved but did not support the existing classification. A high degree of morphological convergence was highlighted and several species appeared best described as species complexes. We therefore proposed a new classification for the subfamily. Our analyses revealed several cases of probable speciation on the same host trees (up to 8 closely related species on one single tree of F. sumatrana ), which raises the question of how resource partitioning occurs to avoid competitive exclusion. Comparisons of our results with fig phylogenies showed that, despite sycoecines being internally ovipositing wasps host-switches are common incidents in their evolutionary history. Finally, by studying the evolutionary properties of the markers we used and profiling their phylogenetic informativeness, we predicted their utility for resolving phylogenetic relationships of Chalcidoidea at various taxonomic levels

Evolution of Fruit Traits in Ficus Subgenus Sycomorus (Moraceae): To What Extent Do Frugivores Determine Seed Dispersal Mode?

Fig trees are a ubiquitous component of tropical rain forests and exhibit an enormous diversity of ecologies. Focusing on Ficus subgenus Sycomorus, a phenotypically diverse and ecologically important Old World lineage, we examined the evolution of fruit traits using a molecular phylogeny constructed using 5 kilobases of DNA sequence data from 63 species (50% of global diversity). In particular, we ask whether patterns of trait correlations are consistent with dispersal agents as the primary selective force shaping morphological diversity or if other ecological factors may provide a better explanation? Fig colour, size and placement (axial, cauliflorous, or geocarpic) were all highly evolutionarily liable, and the same fruit traits have evolved in different biogeographic regions with substantially different dispersal agents. After controlling for phylogenetic autocorrelation, we found that fig colour and size were significantly associated with fig placement and plant-life history traits (maximum plant height and leaf area, respectively). However, contrary to prevailing assumptions, fig placement correlated poorly with known dispersal agents and appears more likely determined by other factors, such as flowering phenology, nutrient economy, and habitat preference. Thus, plant life-history, both directly and through its influence on fig placement, appears to have played a prominent role in determining fruit traits in these figs

The Chalcidoidea bush of life: evolutionary history of a massive radiation of minute wasps.

Chalcidoidea are mostly parasitoid wasps that include as many as 500 000 estimated species. Capturing phylogenetic signal from such a massive radiation can be daunting. Chalcidoidea is an excellent example of a hyperdiverse group that has remained recalcitrant to phylogenetic resolution. We combined 1007 exons obtained with Anchored Hybrid Enrichment with 1048 ultra-conserved elements (UCEs) for 433 taxa including all extant families, >95% of all subfamilies, and 356 genera chosen to represent the vast diversity of the superfamily. Going back and forth between the molecular results and our collective knowledge of morphology and biology, we detected bias in the analyses that was driven by the saturation of nucleotide data. Our final results are based on a concatenated analysis of the least saturated exons and UCE datasets (2054 loci, 284 106 sites). Our analyses support an expected sister relationship with Mymarommatoidea. Seven previously recognized families were not monophyletic, so support for a new classification is discussed. Natural history in some cases would appear to be more informative than morphology, as illustrated by the elucidation of a clade of plant gall associates and a clade of taxa with planidial first-instar larvae. The phylogeny suggests a transition from smaller soft-bodied wasps to larger and more heavily sclerotized wasps, with egg parasitism as potentially ancestral for the entire superfamily. Deep divergences in Chalcidoidea coincide with an increase in insect families in the fossil record, and an early shift to phytophagy corresponds with the beginning of the "Angiosperm Terrestrial Revolution". Our dating analyses suggest a middle Jurassic origin of 174 Ma (167.3-180.5 Ma) and a crown age of 162.2 Ma (153.9-169.8 Ma) for Chalcidoidea. During the Cretaceous, Chalcidoidea may have undergone a rapid radiation in southern Gondwana with subsequent dispersals to the Northern Hemisphere. This scenario is discussed with regard to knowledge about the host taxa of chalcid wasps, their fossil record and Earth's palaeogeographic history

Phylogeny and evolution of life-history strategies in the Sycophaginae non-pollinating fig wasps (Hymenoptera, Chalcidoidea)

<p>Abstract</p> <p>Background</p> <p>Non-pollinating Sycophaginae (Hymenoptera, Chalcidoidea) form small communities within <it>Urostigma </it>and <it>Sycomorus </it>fig trees. The species show differences in galling habits and exhibit apterous, winged or dimorphic males. The large gall inducers oviposit early in syconium development and lay few eggs; the small gall inducers lay more eggs soon after pollination; the ostiolar gall-inducers enter the syconium to oviposit and the cleptoparasites oviposit in galls induced by other fig wasps. The systematics of the group remains unclear and only one phylogeny based on limited sampling has been published to date. Here we present an expanded phylogeny for sycophagine fig wasps including about 1.5 times the number of described species. We sequenced mitochondrial and nuclear markers (4.2 kb) on 73 species and 145 individuals and conducted maximum likelihood and Bayesian phylogenetic analyses. We then used this phylogeny to reconstruct the evolution of Sycophaginae life-history strategies and test if the presence of winged males and small brood size may be correlated.</p> <p>Results</p> <p>The resulting trees are well resolved and strongly supported. With the exception of <it>Apocrytophagus</it>, which is paraphyletic with respect to <it>Sycophaga</it>, all genera are monophyletic. The Sycophaginae are divided into three clades: (i) <it>Eukoebelea</it>; (ii) <it>Pseudidarnes</it>, <it>Anidarnes </it>and <it>Conidarnes </it>and (iii) <it>Apocryptophagus</it>, <it>Sycophaga </it>and <it>Idarnes</it>. The ancestral states for galling habits and male morphology remain ambiguous and our reconstructions show that the two traits are evolutionary labile.</p> <p>Conclusions</p> <p>The three main clades could be considered as tribes and we list some morphological characters that define them. The same biologies re-evolved several times independently, which make Sycophaginae an interesting model to test predictions on what factors will canalize the evolution of a particular biology. The ostiolar gall-inducers are the only monophyletic group. In 15 Myr, they evolved several morphological adaptations to enter the syconia that make them strongly divergent from their sister taxa. Sycophaginae appears to be another example where sexual selection on male mating opportunities favored winged males in species with small broods and wingless males in species with large broods. However, some species are exceptional in that they lay few eggs but exhibit apterous males, which we hypothesize could be due to other selective pressures selecting against the re-appearance of winged morphs.</p